Orchideenkultur

Fachbereich => Orchideenvermehrung und Kultur => Aussaat, symbiotisch => Thema gestartet von: lazzaret am 29.Mär.20 um 12:05 Uhr

Titel: Chloraea collicensis
Beitrag von: lazzaret am 29.Mär.20 um 12:05 Uhr
Hello everybody,

It looks like the germination was beginning after 5 weeks. Very happy.

seeds sowed on a malt-agar medium and put in germination in the dark. Fungus strain have been harvested on a sphagnum moss watered with rainwater.

(https://i64.servimg.com/u/f64/18/58/12/07/collic12.jpg) (https://servimg.com/view/18581207/762)
Titel: Re: chloraea collicensis
Beitrag von: Uhu am 29.Mär.20 um 12:57 Uhr
gratulations, that looks very good :thumb
Titel: Re: Chloraea collicensis
Beitrag von: Berthold am 29.Mär.20 um 13:09 Uhr
Zitat von: lazzaret am 29.Mär.20 um 12:05 Uhr
Fungus strain have been harvested on a sphagnum moss watered with rainwater.

Das ist sehr interessant. Ich hätte gedacht, dass auf den nicht sterilen Sphagnum mehrere Pilzarten leben, die den guten Keimpilz mittelfristig verdrängen.
Titel: Re: Chloraea collicensis
Beitrag von: lazzaret am 29.Mär.20 um 13:37 Uhr

Das ist sehr interessant. Ich hätte gedacht, dass auf den nicht sterilen Sphagnum mehrere Pilzarten leben, die den guten Keimpilz mittelfristig verdrängen.
[/quote]

Maybe i got a lot of chance when i did this isolation...
Now i can see sparse rhizoïds, on 2/3 germinating seeds, connected with hyphaes, so i guess the germination is in progression.
Titel: Re: Chloraea collicensis
Beitrag von: lazzaret am 30.Mär.20 um 15:26 Uhr
this fungus, as thought, is reacting with other chloraea species.

(https://i.servimg.com/u/f64/18/58/12/07/parvif10.jpg) (https://servimg.com/view/18581207/763)
(https://i.servimg.com/u/f64/18/58/12/07/barbat12.jpg) (https://servimg.com/view/18581207/764)
(https://i.servimg.com/u/f64/18/58/12/07/gavilu10.jpg) (https://servimg.com/view/18581207/765)
Titel: Re: Chloraea collicensis
Beitrag von: erdorchideen am 25.Aug.21 um 12:11 Uhr
That is most interesting - and corresponds to my experience that many Chloraeas as a group will be well supported by the same fungus strain. Just needs to find that strain. Congratulations.
Did the seeds develop leafs and roots finally?
Titel: Re: Chloraea collicensis
Beitrag von: neil am 26.Aug.21 um 01:38 Uhr
Hello Frank, please see attached file.

B1 is a Ceratobasidium and on the 6th June I sowed various Choraea species and inculcated the plate with B1.  I have germination of the following cylindrostachya, virescens, crispa and gavilu, just starting now
Titel: Re: Chloraea collicensis
Beitrag von: michael z am 17.Sep.21 um 07:37 Uhr
Hi at the moment I run also some trials with Chloraea. I grow them asymbiotically on BM1. Germination is good for alpina and cristata. I hope they will thrive further on this media. Unfortunately it is very difficult to get seed of this awsome species here in Europe.